Optimization of Enzymatic Activities in Malting of Oat
نویسنده
چکیده
Malting is usually carried out on intact barley seed, while hull is still attached to it. In this study, oat grain with and without hull was subjected to controlled germination to optimize its enzymes activity, in such a way that lipase has the lowest and αamylase and proteinase the highest activities. Since pH has a great impact on the activity of the enzymes, the pH of germination media was set up to 3 to 8. In dehulled oats, lipase and α-amylase had the lowest and highest activities in pHs 3 and 6, respectively whereas the highest proteinase activity was evidenced at pH 7 and 4 in the oats with and without hull respectively. While measurements indicated that the effect of hull on the enzyme activities particularly in lipase and amylase at each level of the pH are significantly different, the best results were obtained in those samples in which their hull had been removed. However, since the similar lipase activity in germinated dehulled oat were recorded at the pHs 4 and 5, therefore it was concluded that pH 5 in dehulled oat seed may provide the optimum enzyme activity for all the enzymes. Keywords—Enzyme activity, malting, oat, optimization. .
منابع مشابه
Germination also causes decomposition of carbohydrates and proteins, when glycolytic and proteolytic enzymes are
Malting is usually carried out on intact barley seed, while hull is still attached to it. In this study, oat grain with and without hull was subjected to controlled germination to optimize its enzymes activity, in such a way that lipase has the lowest and αamylase and proteinase the highest activities. Since pH has a great impact on the activity of the enzymes, the pH of germination media was s...
متن کاملEffect of Barley yellow dwarf virus Infection on Yield and Malting Quality of Barley
Barley yellow dwarf (BYD), the most common and widespread virus disease of cereal crops worldwide (14), may be caused by any one of several Barley yellow dwarf viruses (BYDVs) of the family Luteoviridae. Transmitted by at least 25 aphid species (24), these viruses historically have been classified into two subgroups and five strains based on aphid vector specificity and efficiency (31). However...
متن کاملThe effect of magnetized water on the growth and some biochemical parameters of squash (Cucurbita pepo) plants under toxicity of herbicide trifluralin
To investigate the effect of herbicide Trifluralin and the role of magnetized water on enzymatic activities and content of proline and growth substances including gibberellin (GA3) and cytokinin (6- benzyl amino purine), an experiment was conducted using squash (Cucurbita pepo L. var. Shiraz Hybrid F1) seedlings. Four levels of trifluralin (0, 5, 15 and 25 ppm) and irrigation by distilled and m...
متن کاملMorphology, Carbohydrate Distribution, Gene Expression, and Enzymatic Activities Related to Cell Wall Hydrolysis in Four Barley Varieties during Simulated Malting
Many biological processes, such as cell wall hydrolysis and the mobilisation of nutrient reserves from the starchy endosperm, require stringent regulation to successfully malt barley (Hordeum vulgare) grain in an industrial context. Much of the accumulated knowledge defining these events has been collected from individual, unrelated experiments, and data have often been extrapolated from Petri ...
متن کامل