Characterization of single crystals of the large ribosomal particles from Bacillus stearothermophilus.
نویسندگان
چکیده
Single, three-dimensional crystals of the 50 S ribosomal subunit from Bacillus stearothermophilus (strain NCA) have been characterized using a synchrotron X-ray source. The crystals are orthorhombic with unit cell dimensions: a = 350 A, b = 670 A, c = 905 A, and contain at least one 2-fold screw axis. With cooling to -2 degrees C, the large crystals (1.0 mm X 0.2 mm X 0.1 mm) diffract to 15 to 18 A resolution and are stable in the synchrotron beam for several hours. Despite the large cell dimensions, the reflections are readily resolved when the X-ray diffraction patterns are densitometered with a 25 microns faster.
منابع مشابه
Some X-ray Diffraction Patterns from Single Crystals of the Large Ribosomal Subunit from Bacillus stearothermophilus X-ray diffraction patterns of three-dimensional crystals of the large ribosomal subunit from Bacillus stearothemwphilus have been obtained using a synchrotron radiation
X-ray diffraction patterns of three-dimensional crystals of the large ribosomal subunit from Bacillus stearothemwphilus have been obtained using a synchrotron radiation beam. The patterns contain resolved diffraction spots and indicate packing in relatively small unit cells, the dimensions of which have been tentatively determined. The internal order of the crystals, as reflected in these patte...
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ورودعنوان ژورنال:
- Journal of molecular biology
دوره 187 4 شماره
صفحات -
تاریخ انتشار 1986