Determination of Pioglitazone and Glimepiride in Pharmaceutical Formulations and Rat Plasma by Rp-lc

A simple and sensitive reverse phase high performance liquid chromatographic method for the determination of Pioglitazone (PIO) and Glimepiride (GLM) was developed on a Shimadzu Class vp series HPLC system with a phenomenex C18 column (150x4.6mm, 5μ) using a mobile phase mixture containing methanol and ammonium acetate buffer (pH-3.5) in the ratio of 55: 45. The flow rate was 0.5ml/min and effluents were monitored at 252nm and eluted at 5.63min (PIO) and 7.18min (GLM). Calibration curve was plotted with a range from 25-25000ng/ml for PIO and 10-10000ng/ml for GLM. The assay was validated for the parameters like accuracy, precision, robustness and system suitability parameters. The drugs were extracted from rat plasma by simple liquid-liquid extraction using diethyl-ether as extraction solvent. The results were found to be satisfactory and the method can be adapted for the routine quality control of the drugs in formulations and clinical samples.