Rapid diagnosis of alpha-thalassemia-1 of southeast Asia type and hydrops fetalis by polymerase chain reaction.
نویسندگان
چکیده
A = 5'GCGATCTGGGCTCTGTGTTCT-3' complications, such as hydramnios, preeclampsia, antepartum or postpartum hemorrhage, and difficult vaginal delivery? There is also considerable emotional strain for the mothers and their family members. We use polymerase chain reaction (PCR) and prenatal diagnosis from chorionic villi biopsy samples to identify couples at risk of conceiving fetuses afflicted with Hb Bart's hydrops fetalis. DNA was obtained from the cord blood of six hydrops fetalis
منابع مشابه
Single-tube multiplex-PCR screen for common deletional determinants of alpha-thalassemia.
Alpha-thalassemia is very common throughout all tropical and subtropical regions of the world. In Southeast Asia and the Mediterranean regions, compound heterozygotes and homozygotes may have anemia that is mild to severe (hemoglobin [Hb] H disease) or lethal (Hb Bart's hydrops fetalis). We have developed a reliable, single-tube multiplex-polymerase chain reaction (PCR) assay for the 6 most fre...
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Alpha-thalassemia-1 Southeast Asian (SEA) type is the most common genetic disorder in the Asian population. Couples who are both carriers have a 25% chance of conceiving Bart's hydrops fetalis. Therefore, results from carrier screening and prenatal diagnosis frequently need to be available rapidly. A rapid technique for diagnosis of alpha-thalassemia-1 SEA type was implemented. The technique us...
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Genetic mutations of the alpha genes are common worldwide. In Asia and particularly Southeast Asia, they can result in clinically significant types of alpha-thalassemia, namely hemoglobin (Hb) H disease and Hb Bart's hydrops fetalis. The latter is generally a fatal intrauterine condition, while Hb H disease results in clinical complications that are frequently overlooked. The high prevalence of...
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ورودعنوان ژورنال:
- Blood
دوره 78 3 شماره
صفحات -
تاریخ انتشار 1991