A new mode to light up an adjacent DNA-scaffolded silver probe pair and its application for specific DNA detection.
نویسندگان
چکیده
By fluorescence enhancement of a proximity-dependent DNA-scaffolded silver nanocluster probe pair and exonuclease III-mediated signal amplification, we present a new fluorescence turn-on mode and its application for specific DNA detection.
منابع مشابه
A new mode to light up adjacent DNA-scaffolded silver probe pair and its application for specific DNA detection
Reagents. Oligonucleotides were synthesized and purified by Sangon Inc. (Shanghai, China). The sequences of these oligonucleotides are listed in Table S1. E.coli exonuclease III (Exo III) was purchased from Fermentas Inc. (Vilnius, Lithuania). Silver nitrates (AgNO3) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Sodium borohydride (NaBH4) was obtained from Tianlian...
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In situ hybridization (ISH) is a method that uses labeled complementary single strand DNA or RNA to localize specific DNA or RNA sequences in an intact cell or in a fixed tissue section. The main steps of ISH consist of: probe selection, tissue or sample preparation, pre-hybridization treatment, hybridization and washing, detection and control procedure. Probe selection is one of the important ...
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ورودعنوان ژورنال:
- Chemical communications
دوره 50 100 شماره
صفحات -
تاریخ انتشار 2014