Measuring histone and polymerase dynamics in living cells.
نویسندگان
چکیده
order to obtain individual clones in a 96-well plate. For filter paper cloning, dilute cells are plated in large 150-mm dishes and grown until colonies contain 20–50 cells. Trypsin-treated bits of sterile filter paper are then placed on well-isolated colonies for several minutes until they detach, and the paper with some attached cells is transferred to a 96-well plate. Further details on cell cloning are provided elsewhere.
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ورودعنوان ژورنال:
- Methods in enzymology
دوره 375 شماره
صفحات -
تاریخ انتشار 2004