Biochemistry. Second Edition

نویسنده

  • Andrew Costin
چکیده

161 notebook, with careful documentation of procedures and results. The text is complemented with useful diagrams, and helpful hints abound. Where an experiment is potentially dangerous, warnings are given clearly, and precautions explained. Each chapter has a useful bibliography, with references both to standard textbooks and to appropriate original articles. An interesting feature of this text is that the experiments become progressively more involved and require more independence. The student begins with experiments on the spectrophotometric analysis of riboflavin, identification of a dipeptide by paper chromatography, purification of a protein, and enzyme kinetics, and progresses to carbohydrate analysis including thin layer chromatography, lipid analysis including fatty acid isolation, and finally the isolation and characterization of E. coli DNA. This last chapter consists of a short introduction and a reprint of Julius Marmur's classic 1961 paper on DNA isolation (J Molecular Biology 3: 208), which itself is a detailed, well-explained account. The student is then helped to develop a laboratory protocol based directly on this article; experiments on the characteristics of DNA follow. Thus, the student proceeds in the course of the laboratory work from basic, carefully explained experiments, to following a published research paper, a very useful and practical progression. Selected Exercises for the Biochemistry Laboratory also contains an appendix which describes for the instructor how to set up each experiment, including the amount of reagents and equipment needed. Quite simply, this is one of the nicest manuals for a basic biochemistry laboratory now available, and laboratory instructors should be strongly encouraged to examine it for potential use. Biochemistry may be taught at a variety of different levels and certainly the biochemistry major will demand (or at least his instructors will require) a more rigorous treatment of the subject than would a non-major with perhaps only a peripheral interest in the subject. The preface to Biochemistry by Frank B. Arm-strong states: "Biochemistry is for an introductory course designed to accommodate undergraduates from widely varying curricula." Armstrong presents the subject of biochemistry effectively, slowly leading the student through basic concepts in the early chapters, then introducing proteins, carbohydrates, lipids, and nucleic acids, progressing to metabolism, and ending with DNA, hormones, and nutrition. The tone set in the early introductory chapters continues throughout the text; even when dealing with more difficult areas of biochemistry where greater detail or complex pathways are required, Armstrong strives to teach the basic concepts and keep his …

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عنوان ژورنال:
  • The Yale Journal of Biology and Medicine

دوره 56  شماره 

صفحات  -

تاریخ انتشار 1983