Nickel carbonyl inhibition of RNA synthesis by a chromatin-RNA polymerase complex from hepatic nuclei.
نویسندگان
چکیده
Administration of nickel carbonyl to rats inhibited RNA synthesis by a chromatin-RNA polymerase complex prepared from lysed hepatic nuclei. Rats were killed 6 hr after i.v. administration of Ni(CO)4 in LDSO dosage (2.2 mg Ni/100 g body weight). The rate of RNA synthesis by the chromatinRNA polymerase complex from Ni(CO)4-treated rats averaged 0.16 (S.E. = ±0.02, N = 18) ¿/mole cytidine triphosphate-3 H/10 min/g DNA (controls = 0.33 ±0.03, N =
منابع مشابه
Nickel carbonyl inhibition of RNA polymerase activity in hepatic nuclei.
before sacrifice. The nickel carbonyl was injected into a tail vein by means of a 25 @zl Hamilton syringe, as previously de scribed (6, 7). For induction of RNA polymerase, the rats were given phenobarbital (10 mg/100 gm, i.p.) 20 hr before sacrifice. The rats were killed by guillotine and were exsangui nated. The livers were excised and washed in cold 0.32 M sucrose solution. Portions of the l...
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ورودعنوان ژورنال:
- Cancer research
دوره 30 1 شماره
صفحات -
تاریخ انتشار 1970