EHP: Review and Rededication
نویسندگان
چکیده
-When thioguanine-resistant V79 cells are introduced into spinner flasks containing V79 multicell spheroids, the mutant cells attach to the surface of the spheroids. The composite spheroids thus formed consist of external, thioguanineresistant (TGr) and internal, thioguanine-sensitive cells. Cell sorting with a Becton Dickinson FACS II was used to determine the relative position of TGr cells and sensitivity to fluorescent drugs. After 2-4 days, the TGr cells are found internally as well as externally. The initial percentage of TGr cells varies from 1 to 50%, depending on the size of the single-cell inoculum, size of spheroids and frequency of addition of cells during a 24h period. Differential effects of drugs or radiation on external (cycling, oxic) vs internal (non-cycling, hypoxic) cells of composite spheroids can be assayed by simply plating cells trypsinized from spheroids into standard medium or medium with 2 1ug/ml 6-thioguanine, which allows growth of only TGr cells. For example, Adriamycin, which penetrates poorly into spheroids, is preferentially toxic to external cells, and causes a decrease in the percentage of TGr survivors. Irradiation of composite spheroids also causes preferential killing of external (oxic) TGr cells. However, AF-2, a nitrofuran which preferentially kills hypoxic cells, caused an increase in the % of TGr cells. This technique should prove useful in the evaluation of the preferential cytotoxicity of chemotherapeutic agents, alone or in combination.
منابع مشابه
Rededication of service to developing countries.
might think dedication of EHP to the service of environmental health around the world is a broad but insubstantial goal. However, from the first issue of the revised EHP—published on Earth Day, 22 April 1993—to today, we have worked diligently to make that goal a reality and, to that end, many educational and research institutions in developing countries have for the last eight years received t...
متن کاملThe Assessment of Reliability and Validity of Persian Version of the Endometriosis Health Profile (EHP-30)
Background: The Endometriosis Health Profile-30 (EHP-30) is a disease-specific questionnaire to measure the health-related quality of life in patients with endometriosis. The aim of this study was to evaluate the validity and reliability of the Persian version of Endometriosis Health Pro-file (EHP-30) in women with endometriosis referring to three Gynecology Clinics in Tehran, Iran. Met...
متن کاملRecent Changes at Environmental Health Perspectives
changes in the masthead of the journal, reflecting a renewed commitment to publishing the best research and news in the environmental health sciences. In the 4 months since I assumed the role of editor-inchief for EHP, I have added 21 new associate editors and 26 new Editorial Review Board members. Associate editors are responsible for managing the peer review of papers and making a recommendat...
متن کاملEHP Highlights of the Past Year
Approaching the first anniversary of my becoming Editor-in-Chief of EHP, I want to provide an update to our readers and authors about recent events and changes. My first priority is to thank our associate editors and reviewers, who donate their valuable time and expertise to ensure that peer review is fair and rigorous, and who help EHP publish the most credible and important environmental heal...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Environmental Health Perspectives
دوره 102 شماره
صفحات -
تاریخ انتشار 1994