A chamber for the perfusion of in vitro tissue with multiple solutions.
نویسندگان
چکیده
There are currently no practical systems that allow extended regions (>5 mm(2)) of a tissue slice in vitro to be exposed, in isolation, to changes in ionic conditions or to pharmacological manipulation. Previous work has only achieved this at the expense of access to the tissue for recording electrodes. Here, we present a chamber that allows a tissue slice to be maintained in multiple solutions, at physiological temperatures, and preserves the ability to record from the slice. We demonstrate the effectiveness of the tissue bath with respect to minimizing the mixing of the solutions, maintaining the viability of the tissue, and preserving the ability to record from the slice simultaneously.
منابع مشابه
Innovative Methodology A chamber for the perfusion of in vitro tissue with multiple solutions
Thomas MG, Covington JA, Wall MJ. A chamber for the perfusion of in vitro tissue with multiple solutions. J Neurophysiol 110: 269–277, 2013. First published April 10, 2013; doi:10.1152/jn.01039.2012.—There are currently no practical systems that allow extended regions ( 5 mm) of a tissue slice in vitro to be exposed, in isolation, to changes in ionic conditions or to pharmacological manipulatio...
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25 There are currently no practical systems that allow extended regions (> 5 mm) of a 26 tissue slice in vitro to be exposed, in isolation, to changes in ionic conditions or to 27 pharmacological manipulation. Previous work has only achieved this at the expense of 28 access to the tissue for recording electrodes. Here we present a chamber that allows a 29 tissue slice to be maintained in multip...
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ورودعنوان ژورنال:
- Journal of neurophysiology
دوره 110 1 شماره
صفحات -
تاریخ انتشار 2013