Mutants of Citrobacter freundii that transport and utilize melibiose.

نویسندگان

  • N Okazaki
  • X J Xu
  • T Shimamoto
  • M Kuroda
  • T H Wilson
  • T Tsuchiya
چکیده

We have isolated mutants of Citrobacter freundii that can grow on melibiose. Inducible alpha-galactosidase activity and melibiose transport activity were detected in the mutant cells but not in the wild-type cells. We detected a DNA region which hybridized with melB (the gene for the melibiose transporter) DNA of Escherichia coli in the chromosomal DNA of wild-type C. freundii. Protons, but not sodium ions, were found to be the coupling cations for melibiose (and methyl-beta-D-thiogalactoside) transport in the mutant cells.

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عنوان ژورنال:
  • Journal of bacteriology

دوره 180 13  شماره 

صفحات  -

تاریخ انتشار 1998