A Simple and Rapid Method for Isolation of 124 kDa Oat Phytochrome
نویسندگان
چکیده
Rudolf Grimm and Wolfhart Rüdiger Botanisches Institut der Universität München, Menzinger Straße 67, D-8000 München 19, Bundesrepublik Deutschland Z. Naturforsch. 41c, 988—992 (1986); received June 11, 1986 Avena sativa L ., Native Phytochrome, Solubility of Phytochrome Native (124 kDa) phytochrome has been isolated from etiolated oat seedlings in 25% yield. The procedure includes only one chromatographic step namely a hydroxylapatite column, besides several centrifugation steps. Large scale preparations with 3 kg of fresh tissue are completed within 16 h. The purity of isolated phytochrome is as good as or even better than that of previous preparations as deduced from SDS gelelectrophoresis and from the absorbance ratio A ^ l
منابع مشابه
Photophysics and photochemistry of phytochrome, a chromoprotein in plants
The photophysical properties of 124-kDa phytochrome from etiolated oat shoots and some aspects of its photochemical reactivity are reviewed in terms of the working scheme depicted in Fig. 3. A review discussing these results in greater detail with the exception of the interaction of the Pr form of phytochrome with ubiquitin has been recently published in ref. le. INTRODUCTION Phytochrome is a l...
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