Heat-shock proteins as dendritic cell-targeting vaccines--getting warmer.

Enhancing DNA vaccine potency by combining a strategy to prolong dendritic cell life with intracellular targeting strategies.

We have recently shown that intradermal coadministration of DNA encoding Ag with DNA encoding inhibitors of apoptosis, including Bcl-x(L), prolongs dendritic cell (DC) life and thereby enhances the potency of DNA vaccines in vivo. We have also demonstrated that DNA vaccines targeting Ag to subcellular compartments, using proteins such as Mycobacterium tuberculosis heat shock protein 70, calreti...

Antitumor Immunity gp96 Induces Dendritic Cell Maturation and Cell Surface Targeting of Heat Shock Protein

Enhancement of DNA vaccine potency by coadministration of a tumor antigen gene and DNA encoding serine protease inhibitor-6.

Serine protease inhibitor 6 (SPI-6), also called Serpinb9, inhibits granzyme B and thus may provide a method for delaying apoptotic cell death in dendritic cells. We have previously enhanced DNA vaccine potency by targeting antigen to MHC antigen presentation pathways, using proteins such as Mycobacterium tuberculosis heat shock protein 70, calreticulin, domain II of Pseudomonas aeruginosa exot...

Maturation and Antitumor Immunity Protein gp96 Induces Dendritic Cell Cell Surface Targeting of Heat Shock

مطالعه اثر ادجوانت HSP90 بر ایمنی زایی HbsAg در موش بالب‌سی

Abstract Background: Heat shock proteins, (Hsps) are used as adjuvants in vaccines these proteins induce the inflammatory cytokines and the maturation of monocyte MQ to dendritic cells. This study was done to determine the effect of Hsp90 on the immunogenicity of HbSAg in BalbC mice. Materials and methods: In this experimental study we injected Hsp90 β subunit together with HbsAg to 30 balbC mi...