Kinesin-Microtubule Interactions: Transport and Spindle Formation
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چکیده
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منابع مشابه
Cell cycle control of kinesin-mediated transport of Bik1 (CLIP-170) regulates microtubule stability and dynein activation.
CLIPs are microtubule plus end-associated proteins that mediate interactions required for cell polarity and cell division. Here we demonstrate that budding yeast Bik1, unlike its human ortholog CLIP-170, is targeted to the microtubule plus end by a kinesin-dependent transport mechanism. Bik1 forms a complex with the kinesin Kip2. Fluorescently labeled Bik1 and Kip2 comigrate along individual mi...
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Formation of a mitotic spindle occurs almost effortlessly as cells cycle and is essential for chromosome segregation. Early in a cell cycle, the replication of centrosomes or spindle pole bodies at G1/S leaves mother and daughter poles in close association until forced apart into a bipolar arrangement at mitotic onset. Kinesin-like proteins (Klps) generate a variety of forces that contribute to...
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Human Kinesin-12 (hKif15) plays a crucial role in assembly and maintenance of the mitotic spindle. These functions of hKif15 are partially redundant with Kinesin-5 (Eg5), which can cross-link and drive the extensile sliding of antiparallel microtubules. Although both motors are known to be tetramers, the functional properties of hKif15 are less well understood. Here we reveal how single or mult...
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Eckhard Mandelkow* Eva-Maria Mandelkow Max-Planck-Unit for Structural Molecular Biology, Notkestrasse 85, 22607 Hamburg, Germany. *e-mail: [email protected] Kinesin motor proteins generate motion along microtubules. During the past 17 years more than 1600 publications have appeared on kinesin that reveal an increasing variety of members in this superfamily of proteins with many possessing cor...
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The kinesin-microtubule system plays an important role in intracellular transport and is a model system for integrating biomotor-driven transport into microengineered devices. AC electrokinetics provides a novel tool for manipulating and organizing microtubules in solution, enabling new experimental geometries for investigating and controlling the interactions of microtubules and microtubule mo...
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