Microsoft Word - GOI122BF

CA 125 Adenomyosis Immunohistochemistry Abstract To investigate the histogenic and cytogenic localization of CA 125 in patients with adenomyosis, sections from 8 adenomyosis cases were expressed with monoclonal anti-CA 125 antibodies. Consequently, CA 125 proved to be present on the glandular epithelium localized in muscle layers of adenomyosis. S. Kijima, Department of Obstetrics and Gynecology, Shimane Medical University, 89-1, Enya-cho Izumo-City, Shimane Pref. 693 (Japan) Introduction We reported [1] that considerably higher levels of serum CA 125 were observed in patients with adenomyosis and that serum CA 125 could serve as a good marker to differentiate adenomyosis from uterine myoma. We have now used CA 125 for an immu-nohistochemical diagnosis of adenomyosis. Materials and Methods CA 125 levels in 8 patients with adenomyosis and in 1 with serous cystadenocarcinoma of ovary were measured preoperatively, using an excised CA 125 RIA kit. Nonfixed, fresh frozen sections from 8 adenomyosis cases were stained with hematoxylin and eosin. Serial sections were expressed with monoclonal anti-CA 125 antibodies using the avidin-biotin-perox-idase (ABC) technique [2]. The monoclonal anti-CA 125 antibody was purchased from Centocor, Inc., Malvern, Pa., USA. Normal mouse serum instead of primary specific antibody was used as the negative control and a formalin-fixed, paraffinembedded section of a serous cystadenocarcinoma of ovary, which was highly CA 125-reactive, was used as the positive control. The final reaction was achieved with diami-nobenzidine and methylgreen. Results All 8 patients with adenomyosis had serum CA 125 levels in excess of 39 U/ml (range from 43 to 190 U/ml) and the serum CA 125 level in 1 patient with serous cystadenocarcinoma of ovary was 20,000 U/ml preoperatively. In histopathological studies of the adenomyosis, the lesions contained glandular epithelium and closely resembled normal endometrium and stromal cells. The immunohistochemical evidence was similar in all 8 cases. CA 125 was demonstrated immunohistochemically in the glandular epiCA 125 and Adenomyosis