Effects of heme oxygenase-1 in the intestine on the intestinal barrier function of rats with hemorrhagic shock

Objective: This study aimed to investigate effects of heme oxygenase -1 (HO-1) at different concentrations on intestinal barrier function. Methods: Male SD rats (n=50) were assigned into lactococcus lactis expressing HO-1 (LL-HO-1) group (HO group; n=10), Gln group (n=10), lactococcus lactis group (LL group; n=10), zinc protoporphyrin (ZnPP group; n=10) and PBS group (n=10) randomly. Results: When compared with ZnPP group, mortality, Chiu’s score, and MPO activity reduced significantly, while HO-1 content and IL-10 concentration as well as PaO2 and MAP increased significantly in HO group, Gln group, LL group and PBS group (P<0.05) after fluid infusion. When compared with LL group and PBS group, mortality, Chiu’s score and MPO activity reduced significantly, but HO-1 content and IL-10 concentration, PaO2 and MAP increased significantly in HO group and Gln group (P<0.05) after fluid infusion. When compared with Gln group, mortality, Chiu’s score and MPO activity reduced significantly, while HO-1 content and IL-10 concentration, PaO2 and MAP increased significantly in HO group (P<0.05) after fluid infusion. Conclusion: Oral LL-HO-1 and Gln may stimulate HO-1 expression in intestine of rats with hemorrhagic shock, exerting protective effects on intestinal barrier function, which is associated with inhibition of intestinal inflammation and improvement of PaO2 and MAP after fluid infusion.