Electrophysiology of mycoplasma membranes.
نویسندگان
چکیده
The influence of transmembrane ion fluxes on mycoplasma membrane potentials was studied. Fluorescence intensity of potential-sensitive carbocyanine dyes was calibrated vs. electric membrane potential. Potassium and sodium ion diffusion potentials significantly contributed to mycoplasma membrane potential. Chloride ions were obviously freely permeable across mycoplasma membranes. Under growth conditions the mycoplasma membrane potential was estimated to be delta psi = - 80 mV.
منابع مشابه
Phenotypic Characterization of Mycoplasma synoviae Induced Changes in the Metabolic and Sensitivity Profile of In Vitro Infected Chicken Chondrocytes
In infectious synovitis caused by Mycoplasma synoviae chicken chondrocytes (CCH) may come into direct contact with these bacteria that are also capable of invading CCH in vitro. In this study, phenotype microarrays were used to evaluate the influence of Mycoplasma synoviae on the global metabolic activity of CCH. Therefore, CCH were cultured in the presence of 504 individual compounds, spotted ...
متن کاملBinding of plant lectins to mycoplasma cells and membranes.
The binding of iodinated wheat germ agglutinin, Ricinus communis agglutinin, and concanavalin A to mycoplasma cells and membranes was examined. All mycoplasmas studied specifically bound concanavalin A or R. communis agglutinin and, to a lesser degree, wheat germ agglutinin. The binding of lectins to whole cells was similar to that recorded for membranes, suggesting that significant binding on...
متن کاملMycoplasma-associated immunosuppression: effect on hemagglutinin response to common antigens in rabbits.
A Mycoplasma-associated immunosuppression of the primary and secondary hemagglutinin response to a common gram-negative antigen of Escherichia coli (014) was demonstrated in the rabbit when preincubated mixtures of common gram-negative antigen and Mycoplasma arthritidis membranes were injected intravenously. A similar immunosuppression was demonstrated only for the secondary hemagglutinin respo...
متن کاملImmunological analysis of plasma membranes of a T-strain of mycoplasma (Ureaplasma urealyticum).
The cell membranes of a T-strain of mycoplasma, obtained by ultrasonic disruption, were as effective as whole organisms in eliciting metabolism-inhibiting and complement-fixing antibodies. The soluble fraction separated from cell membranes by centrifugation at 35,000 X g showed a minor ability to elicit an antibody response as measured by metabolism inhibition and complement fixation tests. Aft...
متن کاملElectrophoretic patterns of membrane proteins of Mycoplasma.
Cell membranes of Mycoplasma were isolated either by osmotic lysis or by ultrasonic disruption of the organisms. The membranes were dissolved in phenol-acetic acid-water (2:1:0.5, w/v/v), and membrane proteins were separated electrophoretically in polyacrylamide gels containing 5 m urea and 35% (v/v) acetic acid. The electrophoretic patterns of membrane proteins were highly specific for the dif...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Yale Journal of Biology and Medicine
دوره 56 شماره
صفحات -
تاریخ انتشار 1983