Metachromatic Differential Fluorochroming of Living and Dead Ascites Tumor Cells with Acridine Orange* RALPHVINEGAR
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چکیده
Acridine orange (AO) has been used to vitally stain somatic and germinal nuclei in vivo (1, 2) and to differentially fluorochrome living and dead bacteria, yeast, and onion root tip cells. The latter phenomenon was discovered by Strugger, whose book, Fluoreszenzmikroskopie und Mikrobiologie (7), details the technics required for adapting the metafluorochromatic staining as a test of cell viability. Recent papers (3, 6) have questioned the validity of Strugger's test, and the work pre
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Metachromatic differential fluorochroming of living and dead ascites tumor cells with acridine orange.
Acridine orange (AO) has been used to vitally stain somatic and germinal nuclei in vivo (1, 2) and to differentially fluorochrome living and dead bacteria, yeast, and onion root tip cells. The latter phenomenon was discovered by Strugger, whose book, Fluoreszenzmikroskopie und Mikrobiologie (7), details the technics required for adapting the metafluorochromatic staining as a test of cell viabil...
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LTHOUGH FLUORESCENCE microscopy had its beginnings over half a century ago, it has only recently gained widespread usage as a research tool, and practical applications have yet to be fully exploited. Blood has been one of the tissues most extensively studied by fluorochroming,’ but few reports have been published in English. In addition, the previously reported saline dilution technics for supr...
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