Isoenzymes of creatine kinase; separation by acrylamide gel electrophoresis.

A method for the separation of creatine kinase isoenzymes is described utilizing vertical acrylamide slab electrophoresis. This technique combines the advantages of vertical separation methods with the greater resolving power of acrylamide. The inclusion of the coupling enzymes (hexokinase and glucose-6-phosphate dehydrogenase) in the buffer system should theoretically provide optimal concentration of these enzymes at the reaction sites within the gel. Although a variety of tissues are shown to demonstrate “MB” iso­ enzyme activity, preliminary studies indicate that its presence in serum is confined to cases of myocardial infarction.