Lipid bilayer – DNA interaction mediated by divalent metal cations: SANS and SAXD study
نویسندگان
چکیده
The structure of aggregates formed due to DNA interaction with dipalmitoylphosphatidylcholine (DPPC) in presence of Ca and Zn is examined using smallangle synchrotron X-ray diffraction (SAXD) and small-angle neutron scattering (SANS). SAXD detected two structures: L condensed lamellar phase and L lamellar phase with DNA strands intercalated between the adjacent lipid bilayers, without regular packing at low cation concentration (~1 mM). The high concentration of Zn induces a macroscopic phase separation in mixtures. The SANS curves of DPPC+ions vesicles evaluated using the strip function model have shown different modes of cations binding to the DPPC bilayers.
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