Effect of glucose on isocitrate lyase in Phycomyces blakesleeanus.

Isocitrate lyase from Phycomyces blakesleeanus

Isocitrate lyase was purified from Phycomyces blakesleeanus N.R.R.L. 1555(-). The native enzyme has an Mr of 240000. The enzyme appeared to be a tetramer with apparently identical subunits of Mr 62000. The enzyme requires Mg2+ for activity, and the data suggest that the Mg2+-isocitrate complex is the true substrate and that Mg2+ ions act as a nonessential activator. The kinetic mechanism of the...

Studies in carotenogenesis. 12. The effect of temperature and thiamine concentration on carotenogenesis by Phycomyces blakesleeanus.

Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus

The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in 31P NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentration...

Effect of light on several metabolites of carbohydrate metabolism in Phycomyces blakesleeanus.

The concentrations of all metabolites studied, except fructose 1,6-bisphosphate from wild-type Phycomyces blakesleeanus, were light dependent. This photoregulation appears to be independent of the mad gene product(s) and also independent of carotene biosynthesis regulation. However, the photoregulation of glyceraldehyde 3-phosphate, 2-phosphoglycerate, and phosphoenolpyruvate may be assigned to...

Absence of significant light-induced changes in cAMP levels in sporangiophores of Phycomyces blakesleeanus.

We were unable to find transient changes in the amount of cAMP or cGMP that had been proposed to mediate the light-growth response in sporangiophores of Phycomyces blakesleeanus.