Hybrids between human cell lines belonging to different hematopoietic pathways: analysis of HLA and myeloid surface antigens.

The control of gene regulation can be examined by somatic cell hybridization. Hybrids between human cell lines belonging to different hematopoietic lineages provide model Systems for the analysis of the mechanisms governing the expression of cell surface antigens specific for a particular differentiation pathway 13, 4, 121. In this study, the expression of antigens encoded by the human major histocompatibility (HLA) complex and of molecules present on myeloid cell types were analyzed with monoclonal antibodies on two somatic cell hybrids, HP1 and PUTKO 1. B. Materials and Methods