Identification of Escherichia coli through analysis of 16S rRNA and 16S-23S rRNA internal transcribed spacer region sequences
نویسندگان
چکیده
A bacterial strain, designated BzDS03 was isolated from water sample, collected from Dal Lake Srinagar. The strain was characterized by using 16S ribosomal RNA gene and 16S-23S rRNA internal transcribed spacer region sequences. Phylogenetic analysis showed that 16S rRNA sequence of the isolate formed a monophyletic clade with genera Escherichia. The closest phylogenetic relative was Escherichia coli with 99% 16S rRNA gene sequence similarity. The result of Ribosomal database project's classifier tool revealed that the strain BzDS03 belongs to genera Escherichia.16S rRNA sequence of isolate was deposited in GenBank with accession number FJ961336. Further analysis of 16S-23S rRNA sequence of isolate confirms that the identified strain BzDS03 be assigned as the type strain of Escherichia coli with 98% 16S-23S rRNA sequence similarity. The GenBank accession number allotted for 16S-23S rRNA intergenic spacer sequence of isolate is FJ961337.
منابع مشابه
Molecular Identification of Rare Clinical Mycobacteria by Application of 16S-23S Spacer Region Sequencing
Objective(s) In addition to several molecular methods and in particular 16S rDNA analysis, the application of a more discriminatory genetic marker, i.e., 16S-23S internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. In the current study we aimed to apply this sequencing power to conclusive identification of some Iranian clinical ...
متن کاملComparative sequence analysis of 16S-23S rRNA internal transcribed spacers of the genus Geobacillus
The aim of the present study was to analyse sequences of 16S-23S internal transcribed spacer regions of different Geobacillus species, identify structural elements of these regions and evaluate the possibility to use these structural elements in the taxonomy of the genus Geobacillus. GEOBAC-PCR was used in order to obtain 16S-23S rRNA internal transcribed spacer sequences. Certain structural el...
متن کامل16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny.
In the last few years many attempts have been made to differentiate more than 20 Bifidobacterium species. It has been recognized that identification of bifidobacterial species is problematic because of phenetic and genetic heterogeneities. In order to contribute to our understanding of Bifidobacterium taxonomy, we studied Bifidobacterium phylogeny by performing both 16S rRNA and 16S to 23S (16S...
متن کاملThe Molecular Differentiation of Renibacterjum Salmoninarumisolates
The nucleotide sequences of the rRNA genes and the 5' flanking region were determined for R. salmoninarumATCC 33209T from overlapping products generated by PCR amplification from the genomic DNA. Comparisonof the sequences with rRNA genes from a variety of bacteria demonstrated the close relatedness between R.salmoninarum and the high G+C group of the actinobacteria, in particul...
متن کاملGenetic variations of avian Pasteurella multocida as demonstrated by 16S-23S rRNA gene sequences comparison
Pasteurella multocida is known as an important heterogenic bacterial agent causes some severe diseases such as fowl cholera in poultry and haemorrhagic septicaemia in cattle and buffalo. A polymerase chain reaction (PCR) assay was developed using primers derived from conserved part of 16S-23S rRNA gene. The PCR amplified a fragment size of 0.7 kb using DNA from nine avian P. multocida isolates...
متن کامل