Preparation of Polyribosome Aminoacyl-Transfer Ribonucleic Acid
نویسنده
چکیده
A procedure for preparing polyribosome aminoacyl-tRNA free from contamination by supernatant aminoacyl-tRNA and free amino acids is described. Important features of the procedure are the use of acidic buffers to help protect the amino acid-tRNA linkage and the inclusion ofsodium dodecyl sulphate, to inhibit ribonuclease activity. The specific radioactivity of polyribosome aminoacyl-tRNA is high within 30s and reaches a maximumin24min, well ahead ofpolyribosome peptides which, as described byHerrmann et al. (1971), attain maximum specific radioactivity in about 10min.
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