Chromatographic and electrophoretic separation of platelet-clumping substance in rabbit plasma.

The authors previously reported that platelet-clumping substance (SHIMAMOTO and YAMAZAKI, 1958)1) was detected in heparinized plasma from rabbits administered adrenaline or high molecular weight substance such as agar solution and bacterial endotoxin by an intravenous injection.2) The plateletclumping substance also came to be observed in vitro of ter weak acidification of plasma in the presence of calcium ions.3) Though its chemical structure is still obscure, the platelet-clumping substance has properties different from those of adenosine-diphosphate (ADP), thrombin, fibrinogen, adrenaline, serotonin, thrombocyte agglutinating factors4) or ADP cofactor.5) A similar substance was also f ound in the plasma of patients suffering from acute infectious diseases or thrombosis6) and the substance might be related to thrombi-formation in various morbid conditions. So the authors are investigating analysis of the platelet-clumping substance for the genesis of thrombosis and in the present experiments some chemical properties of the platelet-clumping substance of rabbits were studied using column chromatography and electrophoresis.