Comparison of ELISA, direct electron microscopy and antibody capture technique for detection of rotavirus in human stools--an experience in a developing country.

Development of Direct Sandwich ELISA test for Detection of Rotavirus in Clinical Specimen

Background and Aims: Rotaviruses are one of the main causative agents of acute viral gastroenteritis in both young human and animals. Several methods have been used for diagnosis of rotavirus infection. Most of these methods are not easily available in Iran. Methods: In our study attempt was made to develop a method which could replace the commercially imported Kit. Rota virus was purified and...

A Capture ELISA for the Diagnosis of Visceral Leishmaniasis Using a Monoclonal Antibody against a Leishmanial

capture ELISA system was developed for diagnosis of visceral leishmaniasis (VL) using a monoclonal antibody raised against an antigen previously detected in the urine of VL patients. Urine samples from confirmed VL cases from Yemen, Nepal, Spain, Sudan and Brazil were tested in the capture ELISA in comparison with urine samples from endemic and non-endemic areas along with urine samples from pa...

Comparative evaluation of a commercial enzyme-linked immunoassay and solid-phase immune electron microscopy for rotavirus detection in stool specimens.

Using solid-phase immune electron microscopy (SPIEM) as a reference test, we examined 151 stool specimens from infants and young children with acute gastroenteritis for rotavirus detection by a one-step commercial enzyme-linked immunosorbent assay (ELISA) with labeled monoclonal antibody. Of the 83 samples determined to be positive for rotavirus by SPIEM, 82 were detected as positive by the mon...

Comparison of Rotazyme and direct electron microscopy for detection of rotavirus in human stools.

A total of 115 stools were examined for Rotavirus, using direct electron microscopy (EM) and Rotazyme. The overall agreement was 88.7%. Of the negative results, there was 91.95% agreement. Rotazyme reactions of three-plus or more gave a 100% agreement with EM. The Rotazyme test is a useful diagnostic aid in laboratories not capable of performing EM.

Detection of rotavirus by Latex Agglutination Test (Rotales); Comparison with Electron Microscopy and Complement Fixation Test