Evaluation of Enterolert for enumeration of enterococci in recreational waters.
نویسندگان
چکیده
Enterolert (IDEXX Laboratories Inc., Westbrook, Maine), a semiautomated, most probable number method for enumeration of enterococci, was compared with the standard membrane filter method by parallel testing of 138 marine and freshwater recreational bathing water samples. No statistically significant difference and a strong linear correlation were found between methods. Culturing of 501 Enterolert test wells resulted in false-positive and false-negative rates of 5.1 and 0.4%, respectively. Less time for setup, incubation (24 versus 48 h), and reading of Enterolert permits more efficient monitoring of recreational bathing areas.
منابع مشابه
Are the defined substrate-based methods adequate to determine the microbiological quality of natural recreational waters?
Monitoring the microbiological quality of water used for recreational activities is very important to human public health. Although the sanitary quality of recreational marine waters could be evaluated by standard methods, they are time-consuming and need confirmation. For these reasons, faster and more sensitive methods, such as the defined substrate-based technology, have been developed. In t...
متن کاملEnterococci as indicators of Lake Michigan recreational water quality: comparison of two methodologies and their impacts on public health regulatory events.
The frequency of poor-water-quality advisories issued in Milwaukee and Racine, Wisconsin, in the absence of identifiable sources of contamination brought into question the reliability of the present indicator organism, Escherichia coli. Enteroccoci have been suggested as an alternative to E. coli for freshwater monitoring due to their direct correlation to swimmer-associated gastroenteritis. Th...
متن کاملEvaluation of Techniques for Measuring Microbial Hazards in Bathing Waters: A Comparative Study
Recreational water quality is commonly monitored by means of culture based faecal indicator organism (FIOs) assays. However, these methods are costly and time-consuming; a serious disadvantage when combined with issues such as non-specificity and user bias. New culture and molecular methods have been developed to counter these drawbacks. This study compared industry-standard IDEXX methods (Coli...
متن کاملA critical evaluation of a flow cytometer used for detecting enterococci in recreational waters.
The current U. S. Environmental Protection Agency-approved method for enterococci (Method 1600) in recreational water is a membrane filter (MF) method that takes 24 hours to obtain results. If the recreational water is not in compliance with the standard, the risk of exposure to enteric pathogens may occur before the water is identified as hazardous. Because flow cytometry combined with specifi...
متن کاملIsolation and Identification of Enterococcus Species and Determination of Their Susceptibility Patterns Against Antibiotics and Heavy Metals in Coastal Waters of Iran
Recreational waters should be considered as risks for Enterococcal infections in regions with high utilization and long exposure periods. The aim of this study was to investigate the resistance of Enterococci to selected antibiotics and some heavy metals [Pb+2, Fe+2, Zn+2 and Cr+2]. A total of 75 Enterococcal isolates was obtained from 54 bathing and ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 62 10 شماره
صفحات -
تاریخ انتشار 1996