Liquid Chromatographic Determination of Malvidin-3-O-Glucoside and Malvidin 3, 5-O-Diglucoside in Wine Samples by Direct Injection
نویسندگان
چکیده
A method for the determination of malvidin 3-O-glucoside (Oenin), and malvidin 3, 5-O-diglucoside (Malvin) in wines by on line HPLC coupled with UV, and fluorescence detectors is described. With the proposed method the samples were analysed by direct injection without a previous treatment. For method validation, satisfactory recoveries (> 95%) and suitable repeatabilities (within day: R.S.D. (n=6=) < 3%, and between days; R.S.D. (n=6=) < 3%) were achieved. The method was applied to the analysis of commercially available red wines.
منابع مشابه
Reversed-Phase High-Performance Liquid Chromatography with Electrochemical Detection of Anthocyanins
Anthocyanins, flavonoid compounds present in grapes and wines, were determined by reverse-phase high-performance liquid chromatography (RP-HPLC) with electrochemical detection (RP-HPLC-ED). The method developed consists of RP-HPLC gradient elution with voltammetric detection using a glassy carbon electrode after separation in an Inertsil ODS-3V analytical column. Good peak resolution was obtain...
متن کاملEffect of ellagitannins, ellagic acid and volatile compounds from oak wood on the (+)-catechin, procyanidin B1 and malvidin-3-glucoside content of model wines
Background and Aims: During ageing in oak barrels, wine undergoes changes because of the release of polyphenols and other molecules from wood. The aim of this study was to evaluate the influence of some oak wood-derived volatile compounds, ellagic acid and oak wood extracts on the levels of (+)-catechin, procyanidin B1 and malvidin-3-glucoside. Methods and Results: Phenolics and the oak wood de...
متن کاملAnthocyanins Profile of Grape Berries of Vitis amurensis, Its Hybrids and Their Wines
Anthocyanins are responsible for the color of grapes and wine, an important attribute of their quality. Many authors have used anthocyanins profile to classify the grape cultivars and wine authenticity. The anthocyanin profiles of grape berries of Vitis amurensis, its hybrids and their wines were analyzed by HPLC-ESI-MS/MS. The results identified 17 anthocyanins in these grape cultivars, includ...
متن کاملEnzymatic synthesis of [3'-O-methyl-(3)H]malvidin-3-glucoside from petunidin-3-glucoside.
Malvidin-3-glucoside has been labeled by enzymatic synthesis in a single-step experiment. Catechol-O-methyl transferase catalyzed the B-ring methylation of petunidin-3-glucoside, and S-Adenosyl-L-[methyl-(3)H] methionine was the methyl donor. Solid phase extraction and preparative high-performance liquid chromatography were necessary to separate [3'-O-methyl-(3)H]malvidin-3-glucoside from an is...
متن کاملCharacterization and quantification of polyphenols in Amazon grape (Pourouma cecropiifolia Martius).
The phenolic profile of Amazon grape fruit (Pourouma cecropiifolia Martius) was investigated by high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). For this purpose, suitable extraction and liquid chromatographic methods were developed. Anthocyanins, flavonols and chlorogenic acids were found mainly in the peel. Besides the main anthocyanins, i.e. ...
متن کامل