Calcium Phosphate Bioceramics with Tailored Crystallographic Texture for Controlling Cell Adhesion

The orientation distribution of crystalline grains in calcium phosphate coatings produced by pulsed laser deposition was investigated using an X-ray pole-figure diffractometer. Increased laser energy density of a KrF excimer laser in the 4–7 J/cm range leads to the formation of hydroxyapatite grains with the c-axis preferentially aligned perpendicularly to the substrates. This preferred orientation is most pronounced when the plume direction of incidence is normal to the substrate. This crystallographic texture of hydroxyapatite grains in the coatings is associated with the highly directional and energetic nature of the ablation plume. Anisotropic stresses, transport of hydroxyl groups, and dehydroxylation effects during deposition all seem to play important roles in texture development. Studies of mesenchymal stem cell/biomaterial interactions show that the surfaces with an oriented distribution of hydroxyapatite grains promote significantly better cell adhesion than surfaces with random grain distribution.