Gel-Based and Gel-Free Quantitative Proteomics Approaches at a Glance

Evaluation of Computational Platforms for LS-MS Based Label-Free Quantitative Proteomics: A Global View

LC-MS based quantitative shotgun proteomics has gradually replaced traditional two-dimensional gel electrophoresis to become a method of choice for profiling protein composition in a given biological system. There are two major LC-MS based shotgun proteomics approaches commonly employed: stable isotope labeled method and label-free method. (For review on these methods, please see America and Co...

Gel-Free Proteome Analysis Isotopic Labelling Vs. Label-Free Approaches for Quantitative Proteomics

Gel-free and gel-based proteomics in Bacillus subtilis: a comparative study.

The proteome of exponentially growing Bacillus subtilis cells was dissected by the implementation of shotgun proteomics and a semigel-based approach for a particular exploration of membrane proteins. The current number of 745 protein identifications that was gained by the use of two-dimensional gel electrophoresis could be increased by 473 additional proteins. Therefore, almost 50% of the 2500 ...

Quantitative profiling of the membrane proteome in a halophilic archaeon.

We present a large scale quantitation study of the membrane proteome from Halobacterium salinarum. To overcome problems generally encountered with membrane proteins, we established a membrane preparation protocol that allows the application of most proteomic techniques originally developed for soluble proteins. Proteins were quantified using two complementary approaches. For gel-based quantitat...

Using 7 cm immobilized pH gradient strips to determine levels of clinically relevant proteins in wheat grain extracts

The aim of the work was to test a relatively simple proteomics approach based on phenol extraction and two-dimensional gel electrophoresis (2-DE) with 7 cm immobilized pH gradient strips for the determination of clinically relevant proteins in wheat grain. Using this approach, 157 2-DE spots were quantified in biological triplicate, out of which 55 were identified by matrix-assisted laser desor...