Characterisation and mechanism of oxygenation
نویسندگان
چکیده
The formation ofcis-l,2-dihydroxy-l,2,-dihydronaphthalene from naphthalene by naphthalene oxygenase, purified from Corynebacterium renale ATCC 15075, was demonstrated to involve oxidation of a mol NADH and consumption of one mol oxygen. The enzyme contains one g-atom Fe and one FAD. Catalase inhibited product formation and H2O2 could substitute for NADH in the reaction. Superoxide dismutase inhibited enzyme activity when either NADH or H2O2 was present; the generation of superoxide anion on addition of NADH to the enzyme, in the absence of naphthalene, was detected by the nitro blue tetrazolium reduction method. Hydroxyl radical scavengers, ethanol, mannitol and sodium benzoate, inhibited product formation when either NADH or H2O2 was present. Electron spin resonance studies, under aerobic conditions, indicated that iron of the enzyme underwent valence changes during the course of the reaction.
منابع مشابه
Short communication: Morphological and genetic characterisation of selected Contracaecum (Nematoda: Anisakidae) larvae in Iran
متن کامل
Short communication: Morphological and genetic characterisation of selected Contracaecum (Nematoda: Anisakidae) larvae in Iran
متن کامل
Validity of Spo2 in Measuring of Oxygenation Index
Background Pulse-oximetry (Spo2) widely uses as a help tools for therapeutic interventions in the pediatric intensive care units (PICU). The aim of this study was to evaluate the validity of Spo2 in measuring of oxygenation index (OI) in children. Materials and Methods In a descriptive analytical study, 100 patients who were admitted to PICU Tabriz Children's Hospital were studied. Oxygenation ...
متن کاملModelling Tumour Oxygenation, Reoxygenation and Implications on Treatment Outcome
Oxygenation is an important component of the tumour microenvironment, having a significant impact on the progression and management of cancer. Theoretical determination of tissue oxygenation through simulations of the oxygen transport process is a powerful tool to characterise the spatial distribution of oxygen on the microscopic scale and its dynamics and to study its impact on the response to...
متن کاملPreparation, Characterisation and Antimicrobial Activities of Some Novel Nitriles and Imidazolines
Reaction between 5-methyl-3-aminoisoxazole and ?-acetamidophenylsulpho-nylchloride yielded compound 1. Hydrolysis of compound 1 gave a starting compound 4-methoxybenzal-?-5-methyl isoxazol-3-yl-sulphonamido aniline 2. The compound 2 on condensation with different aldehydes and potassium cyanide yielded the nitriles 3a-l. Cyclocondensation between oxazolinone and compound 2 yielded imidazolines ...
متن کامل