Delayed-type hypersensitivity responses to ESAT-6 and MPT64 from Mycobacterium tuberculosis in the guinea pig.
نویسندگان
چکیده
Two antigens from Mycobacterium tuberculosis, ESAT-6 and MPT64, elicited delayed-type hypersensitivity (DTH) skin responses in outbred guinea pigs infected with M. tuberculosis by the aerosol and intravenous routes but not those sensitized with M. bovis BCG or M. avium. The DTH epitope of ESAT-6 was mapped to the C terminus. Nonresponders to the individual antigens were found, but all animals responded to a combination of ESAT-6 and MPT64 or their respective minimal target peptides. Correspondingly, these molecules could form the basis of a new skin test for tuberculosis.
منابع مشابه
ESAT-6 protein for specific Mycobacterium tuberculosis in guinea pigs skin test
Detection of Mycobacterium tuberculosis (MTB) infection by delayed-type hypersensitivity skin test with purified derivative (PPD) antigen is still used in many countries including Thailand. However, it is less specificity due to significant cross-reactivity with non-tuberculosis mycobacteria. Antigen such as early secretory antigenic target (ESAT6) is secreted in the early phase of infection an...
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ورودعنوان ژورنال:
- Infection and immunity
دوره 66 7 شماره
صفحات -
تاریخ انتشار 1998