Some Practical Considerations on Decalcification of Different Types of Bones
نویسندگان
چکیده
This study was carried out to obtain the best technique for preparation of histological sections of bone with minimal artifacts, preserved tissue and high staining affinity. Different bones from adult female rats were gathered and fixed in 10% neutral buffered formalin (10%NBF) then decalcified in 5 different decalcifying agents. Decalcifiers contained strong acids revealed rapid decalcification when compared with that contained weak acids and neutral ethylenediaminetetraacetic acid (E.D.T.A), however, weak acids and E.D.T.A. showed wellpreserved tissue with high staining affinity.
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