The carbohydrate moiety of serum IgM from Atlantic cod (Gadus morhua L.).

نویسندگان

  • Bergljót Magnadóttir
  • Max Crispin
  • Louise Royle
  • Cristina Colominas
  • David J Harvey
  • Raymond A Dwek
  • Pauline M Rudd
چکیده

The carbohydrate moiety of cod serum IgM was analysed using oligosaccharide sequencing techniques. The carbohydrate moiety constituted about 10% of the molecular weight of cod IgM, was associated with the constant region of the heavy chains (Fc), and was composed of N-linked complex type oligosaccharides. Considerable heterogeneity was observed. Sixteen different glycan structures were identified, over 60% were sialylated and 40% contained core fucose. The carbohydrate moiety of cod IgM was shown to provide protection against protease digestion, and partial deglycosylation abolished the antigen binding property of natural cod anti-TNP-BSA antibody.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Comparison of immunoglobulin (IgM) from four fish species

Immunoglobulin (IgM) was isolated from the serum of four fish species, Atlantic salmon (Salmo salar L.), halibut (Hippoglossus hippoglossus L.), haddock (Melanogrammus aeglefinus L.) and cod (Gadus morhua L.) and a comparison made of some physical and biochemical properties. The molecular weight of IgM varied between the different species and between the different analytical methods used. IgM f...

متن کامل

The behavioural and physiological response of Atlantic cod Gadus morhua L. to short-term acute hypoxia

The average rate of swimming speed and the physiological status or stress of individual Atlantic cod Gadus morhua was monitored in response to short-term acute (STA) hypoxia (i.e. partial pressure of oxygen, PO2 , reduced from 20 9 to 4 3 kPa within 1 h at 10° C). The STA hypoxic response of Atlantic cod was associated with a large primary increase (þ29%) and a large secondary decrease ( 54%) i...

متن کامل

The ontogenic development of innate immune parameters of cod (Gadus morhua L.).

The aim of this study was to monitor the ontogenic development of innate immune parameters of cod (Gadus morhua L.) and to determine the presence of maternal IgM. The general protein composition and enzyme activity was also studied. At intervals, samples were collected of fertilized cod eggs and larvae from 3 days after fertilization until 57 days after hatching. Cell lysates were prepared and ...

متن کامل

Growth and muscle cellularity of diploid and triploid Atlantic cod (Gadus morhua Linnaeus, 1758) larvae

Growth and muscle cellularity of diploid and triploid Atlantic cod (Gadus morhua Linnaeus, 1758) larvae Cecilia Campos Vargas*1, Stefano Peruzzi2, Ørjan Hagen1 1 Faculty of Biosciences and Aquaculture, University of Nordland, Bodø, Norway 2 Faculty of Biosciences, Fisheries and Economics, University of Tromsø, Norway Correspondence: Cecilia C Vargas, University of Nordland, Universitetsalleen 1...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Fish & shellfish immunology

دوره 12 3  شماره 

صفحات  -

تاریخ انتشار 2002