Substrate specificity of Epstein-Barr virus thymidine kinase.
نویسندگان
چکیده
Purified recombinant protein encoded by the BXLF-I open reading frame of the Epstein-Barr virus genome has thymidine kinase activity. The substrate behaviors of various nucleosides toward this enzyme were tested. Halogenated deoxyuridines, zidovudine, and bromovinyldeoxyuridine are efficient substrates, while acyclovir and dihydroxypropylmethylguanine are relatively poor substrates for the Epstein-Barr virus thymidine kinase.
منابع مشابه
Characterization of an Epstein-Barr virus-induced thymidine kinase.
Previous work from our laboratory suggested that the selective inhibition of Epstein-Barr virus (EBV) replication by 1-beta-D-arabinofuranosylthymine in human lymphoid cell lines involved the induction of a new thymidine kinase (TK) able to phosphorylate the thymidine analog. We further characterized this enzyme induced in various EBV-positive cell lines after viral genome activation with a com...
متن کاملIdentification and characterization of the conserved nucleoside-binding sites in the Epstein-Barr virus thymidine kinase.
Thymidine kinase (TK), encoded by EBV (Epstein-Barr virus), is an attractive target for antiviral therapy and provides a novel approach to the treatment of EBV-associated malignancies. Despite the extensive use of nucleoside analogues for the treatment of viral infections and cancer, the structure-function relationship of EBV TK has been addressed rarely. In the absence of any structural inform...
متن کاملEpstein-Barr Virus Myocarditis Presenting as Acute Abdomen in a Child: a Case Report
Introduction Epstein-Barr virus (EBV) infection can present with a variety of manifestation. Case Report Here we present a case of a 7 year- old immunocompetent girl who came with acute abdominal pain , had echocardiographic evidence of myocardial dysfunction and finally was diagnosed as a case of serologically proven acute EBV infection. Conclusion...
متن کاملStudy on the association of Epstein - Barr virus with breast cancer in Khorramabad breast cancer patients, Iran
Background: Breast cancer is one of the most common malignancies in the world, and early diagnosis of this cancer is a key factor in its treatment. This cancer is a multi-stage disease, in which viruses can play a role. EBV is known as an important factor in the development of some human cancers. Therefore, this study was conducted to determine the relationship between Epstein-Barr virus, EBV, ...
متن کاملExpression of Epstein-Barr virus in Hodgkin lymphoma Specimens in IRAN.
Background & Objectives: The Epstein-Barr Virus (EBV( is related with various diseases including infectious mononucleosis, Burkitt's lymphoma, Hodgkin's lymphoma, nasopharyngeal carcinoma and post-transplant lymphoprolifrative disorders. The aim of this study was to characterize the association between EBV and Hodgkin's lymphoma through EBERs in situ hybridization (EBER-ISH) in Iranian pat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 38 9 شماره
صفحات -
تاریخ انتشار 1994