An immunological assay for the sigma subunit of RNA polymerase in extracts of vegetative and sporulating Bacillus subtilis.

Loss of the sigma activity of RNA polymerase of Bacillus subtilis during sporulation.

The activity of the sigma subunit of the RNA polymerase of Bacillus subtilis decreases markedly during the first 2 hr of sporulation. Moreover, sigma activity remains deficient throughout the sporulation process and in dormant spores. The time course of changes in RNA polymerase during sporulation indicates that alterations in the core of RNA polymerase occur after the loss of sigma activity. C...

Antibody Directed against Bacillus subtiLis CT Factor Purified by Sodium Dodecyl Sulfate Slab Gel Electrophoresis EFFECT ON TRANSCRIPTION BY RNA POLYMERASE IN CRUDE EXTRACTS OF VEGETATIVE AND SPORULATING CELLS*

Antibody directed against c factor from vegetative Bacillus subtilis was prepared by immunizing a rabbit with denatured cr polypeptide isolated by electrophoresis of partially purified DNA-dependent RNA polymerase on a sodium dodecyl sulfate-polyacrylamide slab gel. Antiserum to (r reacted specifically with native c polypeptide but not with core RNA polymerase as judged by complement fixation a...

Isolation of a new RNA polymerase-binding protein from sporulating Bacillus subtilis.

RNA polymerase was precipitated from extracts of radioactively labeled vegetative and sporulating Bacillus subtilis with antiserum prepared against vegetative core polymerase. The precipitates were solubilized and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Antiserum added to an extract of vegetative B. subtilis precipitated only the known subunits of core RNA polymer...

Replacement of Vegetative s by Sporulation-Specific s as a Component of the RNA Polymerase Holoenzyme in Sporulating Bacillus subtilis

RNA polymerase from sporulating Bacillus subtilis. Purification and properties of a modified form of the enzyme containing two sporulation polypeptides.

A new form of DNA-dependent RNA polymerase termed enzyme III has been purified from sporulating cells of Bacillus subtilis. In addition to the subunits of core RNA polymerase (beta', beta, alpha, and omega), enzyme III contains sporulation-specific polypeptides of 85,000 (P85) and 27,000 (P27) daltons. P85 corresponds to an RNA polymerase-binding protein previously identified by precipitation o...