Incorporation of Glucose-14C, in vitro, into carbohydrates of intestinal mucosae of sarcoma (subcutaneous Yoshida-sarcoma)-bearing rats.

Scrapings of intestinal mucosae from sarcoma (subcutaneous Yoshida-sarcoma) bearing rats were incubated, in vitro, with U-14C-glucose, comparing with those from normal rats. The resulting 14C-labelled products were fractionated into three carbohydrate fractions: polysaccharide-containing fraction (PS); carbohydrate fraction eluted with 50% aqueous ethanol from the charcoal-absorbed substances (Et); carbohydrate fraction eluted with aqueous ethanol-ammonia from the charcoal-absorbed substances (Et-Am). Monosaccharide components in these carbohydrate fractions were separated by paper chromatography after acidic hydrolysis and the radioactivities incorporated into these sugars were determined. The results showed that glucose-14C incorporated much more into monosac charide components of the three carbohydrate fractions from the sarcoma bearing animals than those from normal rats. No remarkable difference was found on the ratios of radioactivities incorporated into the fractions of glucosamine and galactosamine and also on the analyzed three monosaccharides contents between normal and the sarcoma-bearing conditions. The effects of sarcoma on the metabolism of hexosamine-containing carbohydrates were discussed.