Energetic costs of pyrene metabolism in isolated hepatocytes of rainbow trout, Oncorhynchus mykiss.

Functional and energetic characterization of P-gp-mediated doxorubicin transport in rainbow trout (Oncorhynchus mykiss) hepatocytes.

An assessment of energetic costs associated with P-glycoprotein (P-gp)-mediated xenobiotic efflux is important in understanding the energy budgets, tradeoffs, and fitness of organisms inhabiting contaminated environments. Here, a functional characterization and determination of the energetic costs associated with doxorubicin (DOX) efflux was examined in isolated hepatocytes of rainbow trout. Th...

Tissue-specific metabolism of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss) – a comparison between liver and immune organs

Polycyclic aromatic hydrocarbons as inducers of cytochrome P4501A enzyme activity in the rainbow trout liver cell line, RTL-W1, and in primary cultures of rainbow trout hepatocytes.

In order to investigate cell-specific differences in the response of in vitro models to environmental toxicants, we compared the capacity of nine polycyclic aromatic hydrocarbons (PAHs) to induce cytochrome P4501A (CYPIA) in primary rainbow trout (Oncorhynchus mykiss) hepatocytes and a rainbow trout liver cell line, RTL-W1. Induction of CYPIA was estimated from the catalytic activity of 7-ethox...

Exhaustive Steam Distillation Extraction of Aromatic Organics from Rainbow Trout and Water

Exhaustive steam distillation extracted several intermediate molecular weight organic chemicals effectively from rainbow trout, but a high molecular weight organic, benzo[a]pyrene, was not extracted. Fish lipids that do not steam-distill appear to retain extremely lipophilic organics in the boiling flask. Therefore, exhaustive steam distillation should be used cautiously in screening effluents ...

beta-Naphthoflavone and benzo(a)pyrene treatment affect liver intermediary metabolism and plasma cortisol levels in rainbow trout Oncorhynchus mykiss.

To assess the effects of the polycyclic aromatic hydrocarbons (PAHs) beta-naphthoflavone (beta-NF) and benzo(a)pyrene (BaP) on liver intermediary metabolism and plasma steroid hormones, immature female rainbow trout (Oncorhynchus mykiss) were intraperitoneally injected (2 microl g(-1)) with vegetable oil alone (control) or containing beta-NF or BaP (10 mg kg(-1)) and returned to their tanks; 3,...