Theophylline IMAGEtags (Intracellular Multi Aptamer Genetic tags): the development and evaluation of an RNA reporter system based on the theophylline aptamer
نویسنده
چکیده
iv CHAPTER 1. GENERAL INTRODUCTION 1 Current reporter genes 1 Fluorescent proteins 3 In Vivo Imaging 6 RNA Imaging 8 Aptamers 9 Aptamer structure and stabilization 10 Applications of aptamers 11 Riboswitches natural RNAs with aptamer-like properties 12 Application of aptamers for imaging 13 Sulforhodamine-B and fluorescein aptamers 13 Fluorescence switches and “Light-up” aptamers 14 Theophylline aptamer for imaging 17 Theophylline 17 The theophylline aptamer 19 The IMAGEtag reporter system 22 Collaborator contributions 22 CHAPTER 2. CLONING OF MULTIPLE TANDEM REPEATS OF PROTEIN DOMAINS AND APTAMERS 24 Abstract 24 Introduction 25 Materials and Methods 27 Materials 27 Cloning of initial repetitive sequences from synthetic oligonucleotides 27 Construction of longer repetitive sequences 28 Results and Discussion 28 Repetitive aptamer sequences constructed using oligonucleotides 28 Multiplication of the original repetitive sequences 29 Other repetitive constructs 31 Stability of repetitive constructs 31 Summary 32
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