Important roles of the hyphenated HPLC-DAD-MS-SPE-NMR technique in metabonomics.

Metabolite identification is a key step for metabonomics study. A fully automated hyphenation of HPLC-diode-array detector (DAD) mass spectrometry (MS) solid phase extraction (SPE)-NMR spectroscopy (HPLC-DAD-MS-SPE-NMR) is one of the most efficient methods to determine the structure of a given unknown metabolite in a complex mixture (metabonome) and hence represents one of the most important analytical techniques for the further development of metabonomics. In this review, some recent applications of this technique in identifying novel and trace metabolites in plant extracts and drug metabolism have been discussed. Modification of this hyphenated technique, enabling multiple trappings of strong polar metabolites for biofluids, needs further development.