In vivo metabolic analysis of Pseudomonas aeruginosa live bacteria using High Resolution Magic Angle Spinning NMR spectroscopy

Ultrafast high-resolution magic-angle-spinning NMR spectroscopy.

We demonstrate the acquisition of ultrafast 2D NMR spectra of semi-solid samples, with a high-resolution magic-angle-spinning setup. Using a recent double-quantum NMR pulse sequence in optimised synchronisation conditions, high-quality 2D spectra can be recorded for a sample under magic-angle spinning. An illustration is given with a semi-solid sample of banana pulp.

Discrimination of Basal Cell Carcinoma from Normal Skin Tissue Using High-Resolution Magic Angle Spinning 1H NMR Spectroscopy

High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy is a useful tool for investigating the metabolism of various cancers. Basal cell carcinoma (BCC) is the most common skin cancer. However, to our knowledge, data on metabolic profiling of BCC have not been reported in the literature. The objective of the present study was to investigate the metabolic profil...

Evaluation of high resolution magic-angle coil spinning NMR (HR-MACS-NMR) spectroscopy for metabolic profiling of nanoliter tissue biopsies

HRMAS NMR Spectroscopy - An Important Tool in Understanding Joint Degradation in Osteoarthritis

+Shet, K; Siddiqui, M S; Kurhanewicz, J; Ries, M; Yoshihara, H; Li, X +Deaprtment of Radiology, Department of Orthopaedic Surgery, University of California, San Francisco, CA, USA [email protected] Introduction It is well documented that significant biochemcial changes take place in cartilage matrix at early stages of osteaorthritis. The recent developments in high-field NMR spect...

Structural Masquerade of Plesiomonas shigelloides Strain CNCTC 78/89 O-Antigen—High-Resolution Magic Angle Spinning NMR Reveals the Modified d-galactan I of Klebsiella pneumoniae

The high-resolution magic angle spinning nuclear magnetic resonance spectroscopy (HR-MAS NMR) analysis of Plesiomonas shigelloides 78/89 lipopolysaccharide directly on bacteria revealed the characteristic structural features of the O-acetylated polysaccharide in the NMR spectra. The O-antigen profiles were unique, yet the pattern of signals in the, spectra along with their ¹H,13C chemical shift...