Specific activities and transcript levels of glycolytic enzymes under dehydration in chickpea (Cicer arietinum L.) seedlings
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چکیده
The effect of dehydration on the specific activities and transcript levels of several glycolytic enzymes was determined in two chickpea (Cicer arietinum L.) cultivarsPusa362 (drought tolerant) and SBD377 (drought sensitive). Dehydration treatment was imposed on 6 days old chickpea seedlings by removing them from pots and keeping them on table top on 3MM Whatman paper at room temperature for 5 h. The stress imposed was judged by determining relative water content (RWC) and proline content. RWC decreased and proline content accumulated after five hours of dehydration treatment. The decline in RWC was higher in SBD377 than in Pusa362, while varietal differences were absent in proline content. With the exception of NADP-glyceraldehyde-3-phospahte dehydrogense, whose activity as well as transcript level showed marginal decrease in both the varieties, dehydration had little effect on all glycolytic enzymes studied.
منابع مشابه
Long term transcript accumulation during the development of dehydration adaptation in Cicer arietinum.
Cool season crops face intermittent drought. Exposure to drought and other abiotic stresses is known to increase tolerance of the plants against subsequent exposure to such stresses. Storage of environmental signals is also proposed. Preexposure to a dehydration shock improved adaptive response during subsequent dehydration treatment in a cool season crop chickpea (Cicer arietinum). We have ide...
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