Isolation and Identification of Acetogenic and Methanogenic Bacteria from Anoxic Black Sediments and Their Role in Biogas Production

The increasing population and developing science and technology increased human comfort and needs which in turn increased need of burning fuel. Universal resource of this fuel in non-renewable therefore there is a constant search for renewable eco-friendly energy source. Biogas is one such energy which needs greater attention towards effective production of methane under anaerobic decomposition. In the light of this the present studies aimed at isolating the acetogenic and methanogenic bacterial species and understand their growth dynamics for enrichment of biogas production. Acetobacterium woodii and Methanospirillum hungatei were the acetogenic and methanogenic species which reported to be effectively involved in anaerobic decomposition. Present study was successfully identifying the species through specific media and characterizes them biochemically. Growth of acetogenic and methanogenic species found to be enhanced by yeast extract and increasing substrate at acidic pH for acetogenic species and alkaline pH for methanogenic species. However, growth was higher with methanogenic species when compared to the acetogenic species in concentration of yeast extract and substrate. During the biogas production enrichment of both the species increased the biogas production both at individual and combined levels. The present study would suggest that detailed study on this species at different concentration of inoculate, temperature, and other chemical and physical resources would further strengthen the attempt in effective production of biogas through anaerobic digestion.