Functional diversity of chitin synthases of Aspergillus nidulans in hyphal growth, conidiophore development and septum formation.
نویسنده
چکیده
Fungal chitin synthases are classified into seven classes. Recent advances in genome sequence analyses have revealed that there are more than seven chitin synthase-encoding genes in the genome of filamentous ascomycete fungi. To clarify the functional differences among these genes in the morphogenesis and hyphal tip growth, we have cloned six chitin synthase-encoding genes from Aspergillus nidulans and analyzed their functions. The results obtained suggest that chitin synthases are structurally and functionally divergent and play crucial roles in the growth and morphogenesis of A. nidulans.
منابع مشابه
The Neurospora rca-1 gene complements an Aspergillus flbD sporulation mutant but has no identifiable role in Neurospora sporulation.
The Aspergillus nidulans flbD gene encodes a protein with a Myb-like DNA-binding domain that is proposed to act in concert with other developmental regulators to control initiation of conidiophore development. We have identified a Neurospora crassa gene called rca-1 (regulator of conidiation in Aspergillus) based on its sequence similarity to flbD. We found that N. crassa rca-1 can complement t...
متن کاملCalcineurin localizes to the hyphal septum in Aspergillus fumigatus: implications for septum formation and conidiophore development.
A functional calcineurin A fusion to enhanced green fluorescent protein (EGFP), CnaA-EGFP, was expressed in the Aspergillus fumigatus DeltacnaA mutant. CnaA-EGFP localized in actively growing hyphal tips, at the septa, and at junctions between the vesicle and phialides in an actin-dependent manner. This is the first study to implicate calcineurin in septum formation and conidiophore development...
متن کاملInteraction between developmental and cell cycle regulators is required for morphogenesis in Aspergillus nidulans.
In Aspergillus nidulans, mutation of the transcriptional regulator brlA arrests formation of asexual spore-forming structures called conidiophores but does not hinder vegetative hyphal growth. During conidiophore development a 6-fold, brlA-dependent increase in the kinase activities of NIMX(cdc2) and NIMA occurs. A similar level of kinase induction was promoted by ectopic expression of brlA. No...
متن کاملTransportation of Aspergillus nidulans Class III and V Chitin Synthases to the Hyphal Tips Depends on Conventional Kinesin
Cell wall formation and maintenance are crucial for hyphal morphogenesis. In many filamentous fungi, chitin is one of the main structural components of the cell wall. Aspergillus nidulans ChsB, a chitin synthase, and CsmA, a chitin synthase with a myosin motor-like domain (MMD) at its N-terminus, both localize predominantly at the hyphal tip regions and at forming septa. ChsB and CsmA play cruc...
متن کاملhymA (hypha-like metulae), a new developmental mutant of Aspergillus nidulans.
Asexual fruiting body development in Aspergillus nidulans requires a precise spatial and temporal coordinated expression of many genes. Insertional mutagenesis was used to isolate and characterize a new mutant of A. nidulans in which hyphal growth was slightly reduced and conidiophore development was specifically blocked at the metula stage. In contrast to the uninucleate metulae of the wild-ty...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Medical mycology
دوره 47 Suppl 1 شماره
صفحات -
تاریخ انتشار 2009