Freeze-drying of Foot-and-mouth Disease Virus and Storage Stability of the Infectivity of Dried Virus at 4 C.

Freeze-drying of foot and mouth disease virus and its application in inactivated virus vaccine production

Five different subtypes of foot and mouth disease (FMD) virus propagated in BHK21 C13 monolayer cell cultures, namely 05, A5/!0, A22, C¡ and Asia 1/1, were used to study the stability of live virus during freeze-drying and subsequent storage. Binary ethyleneimine-inactivated FMD virus vaccines adjuvanted with purified saponin, "Quil-A", without aluminium hydroxide gel, were examined before and ...

Lyophilizing Foot-and Mouth Disease Virus at Low Drying Temperature

Comparison of two gel filtration chromatography resins for the purification of foot-and-mouth disease virus as a purified vaccine antigen

Introduction: Foot-and-mouth disease (FMD) is a highly contagious and economically devastating viral disease of livestock that is categorized in list A of animal diseases by the World Organisation for Animal Health (OIE). Vaccination is effective against FMD and the vaccine production centers largely use the industrial ultra-filtration and chromatography in order to remove the cellular proteins...

Stability of Southern Bean Mosaic and Carnation Mottle Viruses during the Preservation by Freeze-drying

The infectivity of purified southern bean mosaic virus (SBMV) in 10mM sodium phosphate buffer, pH 7.0, decreased to 11% after freeze-drying. Such partially swollen virions were found to be markedly susceptible to RNase and sodium dodecyl sulfate (SDS). However, in the preparations supplemented with 0.5% lysine prior to freeze-drying the conformational changes of the virions were suppressed and ...

Foot-and-mouth disease virus: stability of neutralizing antibody after freeze-drying and air-drying.

It is possible to stabilize foot-and-mouth disease antiserum by either freeze-drying in bottles or air-drying on paper for periods of 7 months.