Temperature adaptation of Entamoeba histolytica and its effect on virulence.

نویسنده

  • H A CABRERA
چکیده

Cabrera and Porter (1958) found that the maximum culture temperatures for three strains of Entamoeba histolytica (UC, 201 and 202) were 41.0, 41.0, and 41.3”C and the minimum were 32.0, 32.0, and 31.7”C. In the present investigation, by progressive gradual elevation of the culture temperature, these three strains grown in Ringer-Egg-Locke (REL) medium were adapted to 42”C, and one of them to 42.5”C. Similarly, by lowering the temperature, all three strains were adapted to continuous cultivation at 30°C and one of them to 29°C. The adaptation of E. histolytica to high and low temperatures presented a very logical question: Are these adapted strains similar in behavior to the unadapted ones? To find the answer to this question a detailed study of the adapted strains compared with the unadapted strains was undertaken.

منابع مشابه

Identification of S-Nitrosylated (SNO) Proteins in Entamoeba histolytica Adapted to Nitrosative Stress: Insights into the Role of SNO Actin and In vitro Virulence

We have recently reported that Entamoeba histolytica trophozoites can adapt to toxic levels of the nitric oxide (NO) donor, S-nitrosoglutathione (GSNO). Even if the consequences of this adaptation on the modulation of gene expression in NO-adapted trophozoites (NAT) have been previously explored, insight on S-nitrosylated (SNO) proteins in NAT is missing. Our study aims to fill this knowledge g...

متن کامل

Glucose Starvation Boosts Entamoeba histolytica Virulence

The unicellular parasite, Entamoeba histolytica, is exposed to numerous adverse conditions, such as nutrient deprivation, during its life cycle stages in the human host. In the present study, we examined whether the parasite virulence could be influenced by glucose starvation (GS). The migratory behaviour of the parasite and its capability to kill mammalian cells and to lyse erythrocytes is str...

متن کامل

Identification of differentially expressed genes in virulent and nonvirulent Entamoeba species: potential implications for amebic pathogenesis.

Entamoeba histolytica is a protozoan parasite that causes colitis and liver abscesses. Several Entamoeba species and strains with differing levels of virulence have been identified. E. histolytica HM-1:IMSS is a virulent strain, E. histolytica Rahman is a nonvirulent strain, and Entamoeba dispar is a nonvirulent species. We used an E. histolytica DNA microarray consisting of 2,110 genes to asse...

متن کامل

Primary pathogenicity analysis of a Chinese Entamoeba histolytica isolate.

This study is the first to isolate an Entamoeba histolytica strain from Chinese amoebic patients and to conduct a detailed examination of its virulence. A fecal sample that contains cysts of E. histolytica was obtained from Guangxi province. The sample was cultured axenically and then cloned by limiting dilution, and named as XLAC. In vitro and in vivo tests were conducted to evaluate the virul...

متن کامل

Identification of an Entamoeba histolytica serine-, threonine-, and isoleucine-rich protein with roles in adhesion and cytotoxicity.

Entamoeba histolytica is a leading cause of parasitic death globally. However, the molecular framework regulating pathogenesis is poorly understood. We have previously used expression profiling to identify Entamoeba genes whose expressions were strictly associated with virulent strains (R. C. MacFarlane and U. Singh, Infect. Immun. 74:340-351, 2006). One gene, which we have named EhSTIRP (Entam...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

متن کامل
عنوان ژورنال:
  • Experimental parasitology

دوره 7 3  شماره 

صفحات  -

تاریخ انتشار 1958