Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma.
نویسندگان
چکیده
A method was developed to separate and quantitatively determine two different triglyceride lipase activities in human postheparin plasma: hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL). Affinity chromatography on heparin-Sepharose columns was used for the separation. Rechromatography of purified H-TGL on heparin-Sepharose resulted in recoveries of 74 and 97% of these enzyme activities, respectively. The analytical errors for the determinations of the two activities were 11.4 and 9.6%, respectively.
منابع مشابه
A new method for the measurement of lipoprotein lipase in postheparin plasma using sodium dodecyl sulfate for the inactivation of hepatic triglyceride I ipasel
Lipoprotein lipase (LPL) and hepatic triglyceride lipase (H-TGL) are lipolytic activities found in postheparin plasma. A simple and precise method for the direct determination of LPL in postheparin plasma is described. Preincubation of this plasma (45-60 min at 26°C) with sodium dodecyl sulfate (35-50 mM) in 0.2 M Tris-HC1 buffer, pH 8.2, results in the inactivation of H-TGL, while leaving LPL ...
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ورودعنوان ژورنال:
- Journal of lipid research
دوره 18 4 شماره
صفحات -
تاریخ انتشار 1977