Protective Effect of Ficus Religiosa on Cyclophosphamide Induced Oxidative Stress in Brain

Objective: Protective effect of Ficus religiosa leaf extract on cyclophosphamide induced oxidative stress on brain. Material and Method: In vitro antioxidative analysis for the exploration of the antioxidative properties of Ficus religiosa leaf extract (FRL) and Ficus religosa bark (FRB) was done. To analysis the In vivo antioxidant property of extracts on the oxidative stress induced by the cyclophosphamide. Oral administration of leaf and bark extracts (0.1ml/kg of body weight/day) for 7 days prior to an acute dose of cyclophosphamide (75 mg/kg) on 5th day which significantly (P< 0.05) augmented level of malondieldehyde in the rat brain. Later animal were sacrificed and brain was taken out and homogenate were prepared in Tris buffer (pH 7.4) in cold condition. And taken for biochemical estimation involving Superoxide dismutase, Lipid peroxide and Reduced Glutathion test. Result and conclusion: The cyclophosphamide cause declination in the level of glutathione, superoxide dismutase and increase in lipid peroxidation which was with the administration of Ficus religiosa extract significantly (P< 0.05) prevented in brain of rat. Analysis of different biochemical parameters, results clearly indicated the prophylactic action of FRLand FRB extract against cyclophosphamide induced oxidative stress in brain.