Evolutionary Proteomics: Empowering Tandem Mass Spectrometry and Bioinformatics Tools for the Study of Evolution
نویسندگان
چکیده
Irving E. Vega1, Dan Rittschof2, Gary H. Dickinson3 and Ian Musgrave4 1Department of Biology and Protein Mass Spectrometry Core Facility, College of Natural Science, University of Puerto Rico – Río Piedras Campus, Puerto Rico, 2MSC Division, Nicholas School and Biology Department Duke University, 3Department of Oral Biology, University of Pittsburgh School of Dental Medicine, 4Discipline of Pharmacology, School of Medical Sciences, University of Adelaide, Adelaide, 1,2,3USA 4Australia
منابع مشابه
Proteome analysis of Cryptosporidium parvum and C. hominis using two-dimentional electrophoresis, image analysis and tandem mass spectrometry
Until recently, Cryptosporidium was thought to be a single species genus. Molecular studies now showthat there are at least 10 valid species of this parasite. Among them, two morphologically identical species, C.hominis and C. parvum are the most pathogenic identified to date and share 97% of identical genomes.Post-genomic analyses is therefore necessary to explore further the...
متن کاملData analysis and bioinformatics tools for tandem mass spectrometry in proteomics.
Data processing is a central and critical component of a successful proteomics experiment, and is often the most time-consuming step. There have been considerable advances in the field of proteomics informatics in the past 5 years, spurred mainly by free and open-source software tools. Along with the gains afforded by new software, the benefits of making raw data and processed results freely av...
متن کاملPIUS: peptide identification by unbiased search
SUMMARY We present PIUS, a tool that identifies peptides from tandem mass spectrometry data by analyzing the six-frame translation of a complete genome. It differs from earlier studies that have performed such a genomic search in two ways: (i) it considers a larger search space and (ii) it is designed for natural peptide identification rather than proteomics. Differently from other peptidomics ...
متن کاملGraph-based peak alignment algorithms for multiple liquid chromatography-mass spectrometry datasets
UNLABELLED Liquid chromatography coupled to mass spectrometry (LC-MS) is the dominant technological platform for proteomics. An LC-MS analysis of a complex biological sample can be visualized as a 'map' of which the positional coordinates are the mass-to-charge ratio (m/z) and chromatographic retention time (RT) of the chemical species profiled. Label-free quantitative proteomics requires the a...
متن کاملGrid-based Analysis of Tandem Mass Spectrometry Data in Clinical Proteomics
Biomarker detection is one of the greatest challenges in Clinical Proteomics. Today, great hopes are placed into tandem mass spectrometry (MS/MS) to discover potential biomarkers. MS/MS is a technique that allows large scale data analysis, including the identification, characterization, and quantification of molecules. Especially the identification process, that implies to compare experimental ...
متن کامل