Extracts Attenuates Intestinal Barrier Dysfunction
نویسندگان
چکیده
Maintenance of intestinal barrier integrity is crucial for preventing inflammatory bowel 15 diseases (IBDs) onset and exacerbations. In this work we study the effect of a fixed combination of 16 Lactobacillus reuteri and Lactobacillus acidophilus and herbal extracts in an in vitro inflammation 17 experimental model. Caco-2 cell monolayer was exposed to INF-γ+TNF-α or to LPS; Trans Epithelial 18 Electrical Resistance (TEER) and paracellular permeability were investigated. ZO-1 and occludin 19 tight junctions (TJs) were also investigated by mean of immunofluorescence. The pre-treatment with 20 the fixed combination of probiotics and herbal extracts prevented the inflammation-induced TEER 21 decrease, paracellular permeability increase and TJs translocation. In summary the fixed 22 combination of probiotics and herbal extracts investigated in this research was found to be an 23 interesting candidate for targeting the re-establishment of intestinal barrier function in IBDs 24 conditions. 25
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